monoclonal antibodies mouse anti human αeβ7 cd103 unconjugated  (Bio-Rad)

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Cell surface expression of ICAM-1 (6.5β5 mAb), E-selectin (BBIG-E4 mAb) and E-cadherin (E4.6 mAb: αeβ7 binding site and HECD-1: homotypic binding site) on oral (H357 and H376) and skin (UP) keratinocyte cell lines and breast cell line (16E6.A5) quantified by cell-based ELISA; n = 6.

Article Snippet: Monoclonal antibodies Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Expressing, Binding Assay, In-Cell ELISA

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-positive H357 oral keratinocyte cell line. Lymphocytes were incubated with or without antibodies against αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). Essentially similar results were found with the skin cell line UP – results not shown. For each experiment n = 6 or above.

Article Snippet: Monoclonal antibodies Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated and TGF-β-activated PBL to the breast cell line 16E6.A5. Lymphocytes were incubated either with or without antibodies against the homotypic binding site on E-cadherin (HECD-1), the αeβ7 binding site on E-cadherin (E4.6), LFA-1 (TS1/18) and αeβ7 (BerACT8). For each experiment n = 6 or above.

Article Snippet: Monoclonal antibodies Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-negative oral keratinocytes cell line H376. Lymphocytes were incubated either with or without antibodies to αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). For each experiment n = 6 or above.

Article Snippet: Monoclonal antibodies Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Cell surface expression of ICAM-1 (6.5β5 mAb), E-selectin (BBIG-E4 mAb) and E-cadherin (E4.6 mAb: αeβ7 binding site and HECD-1: homotypic binding site) on oral (H357 and H376) and skin (UP) keratinocyte cell lines and breast cell line (16E6.A5) quantified by cell-based ELISA; n = 6.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Expressing, Binding Assay, In-Cell ELISA

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-positive H357 oral keratinocyte cell line. Lymphocytes were incubated with or without antibodies against αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). Essentially similar results were found with the skin cell line UP – results not shown. For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated and TGF-β-activated PBL to the breast cell line 16E6.A5. Lymphocytes were incubated either with or without antibodies against the homotypic binding site on E-cadherin (HECD-1), the αeβ7 binding site on E-cadherin (E4.6), LFA-1 (TS1/18) and αeβ7 (BerACT8). For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-negative oral keratinocytes cell line H376. Lymphocytes were incubated either with or without antibodies to αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay